Comparative ultrastructure of Mycobacterium leprae and Mycobacterium lepraemurium cell envelopes.

Organized nerve tissue cultures infected with Mycobacterium leprae and Mycobacterium lepraemurium.

Modification of the fluorescent staining method for mycobacterial cells.

The fluorescein diacetate-ethidium bromide (FDA/EB) staining method for determining the viability of mycobacterial cells reported by Kvach and Veras was modified for applying the method to Mycobacterium leprae and Mycobacterium lepraemurium. Our method clearly differentiated green-stained cells from red-orange ones with either mycobacterial species and permitted their original fluorescence for ...

Application of diffusion chamger technic to the cultivation of Mycobacterium lepraemurium. I. In vivo studies.

Numerous studies have so far been conducted on the cultivation of Mycobacterium leprae and Mycobacterium lepraemurium. However, no media-bound method of cultivation has been established. A number of investigators have reported that M. lepraemurium may be cultivated by cell culture technics. The method employed by Chang (1. 2. 3 ), Chang and Neikirk (4), Chang, Anderson and Vaituzis (~), in whic...

Catalases, peroxidases, and superoxide dismutases in Mycobacterium leprae and other mycobacteria studied by crossed immunoelectrophoresis and polyacrylamide gel electrophoresis.

The five mycobacteria Mycobacterium lepraemurium, M. leprae, M. bovis BCG, M. smegmatis, and M. intracellulare were studied. Catalase and peroxidase activities were demonstrated in polyacrylamide and crossed immunoelectrophoresis gels for M. lepraemurium, M. intracellulare, and BCG, but not for M. leprae. Peroxidase and catalase activities were associated with the same precipitate line in cross...

Insights from the Genome Sequence of Mycobacterium lepraemurium: Massive Gene Decay and Reductive Evolution

Mycobacterium lepraemurium is the causative agent of murine leprosy, a chronic, granulomatous disease similar to human leprosy. Due to the similar clinical manifestations of human and murine leprosy and the difficulty of growing both bacilli axenically, Mycobacterium leprae and M. lepraemurium were once thought to be closely related, although it was later suggested that M. lepraemurium might be...